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Philips CM12 (S)TEM Alignment & Setup Manual
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Erwin Sabio Philips CM12 Alignment & Setup Manual Page 2
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Erwin Sabio Philips CM12 Alignment & Setup Manual Page 3 Table of Contents Parts of the CM12 4 The Microscope The Control Panels The Specimen Holder The Side Entry Specimen Chamber Start-up Proced...
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Erwin Sabio Philips CM12 Alignment & Setup Manual Page 4 Parts of the CM12 The Microscope Figure 1 The Philips CM12 (S)TEM Microscope 1 Electron Gun A filament tip (La B6 in this case) that emits ...
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Erwin Sabio Philips CM12 Alignment & Setup Manual Page 5 The Control Panels Left Control Panel Figure 2 The Control Panel located to the left of the microscope column. Right Control Panel Figure 3...
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Erwin Sabio Philips CM12 Alignment & Setup Manual Page 6 The Specimen Holder Figure 4 The CM12 Specimen Holder 22 Black Cap 24 Specimen Cavity & Clamp 23 O-ring The Side Entry Specimen Chamber...
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Erwin Sabio Philips CM12 Alignment & Setup Manual Page 7 Powering Up the Microscope 1. Start by logging in to the computer connected to the TEM. Login is a necessary step to prevent unauthorized u...
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Erwin Sabio Philips CM12 Alignment & Setup Manual Page 8 3. Start saturating the filament by slowly turning the Filament Knob [18] clockwise. Each click raises the level by one with a wait time of...
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Erwin Sabio Philips CM12 Alignment & Setup Manual Page 9 Column Alignment Centering the Beam 1. To center the beam more easily, adjust the intensity knob [9] to find the cross over i.e. the bright...
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Erwin Sabio Philips CM12 Alignment & Setup Manual Page 10 Condenser Aperture Adjustment 1. The condenser aperture controls the beam that passes through the condenser lens. If the aperture is not a...
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Erwin Sabio Philips CM12 Alignment & Setup Manual Page 11 Sample Loading & Unloading Mounting the Sample 1. Samples for the CM12 are deposited as thin films on a copper mesh grid such as in Fi...
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Erwin Sabio Philips CM12 Alignment & Setup Manual Page 12 Sample Image Adjustment-Optimizing Eucentric (Z) Height 1. Adjust the beam intensity on the sample or increase the magnification by turnin...
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Erwin Sabio Philips CM12 Alignment & Setup Manual Page 13 5. Once rotation center has been aligned, press ALGN to exit the current mode. Sample Image Adjustment-Centering the Pivot Point 1. Adjust...
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Erwin Sabio Philips CM12 Alignment & Setup Manual Page 14 Obtaining the Image Fine Tuning the Microscope 1. Use the mechanical stage controls to select certain areas of the sample to be captured. ...
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Erwin Sabio Philips CM12 Alignment & Setup Manual Page 15 Power Down Sequence 1. Return the viewing screen back to its original position and spread the beam. 2. If high magnification was used, low...
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Erwin Sabio Philips CM12 Alignment & Setup Manual Page 16 Photo Credits Figure # Source 1 Mehraeen S. (2007, January 19). 230L, first report. Email attachment. 2 Browning, Nigel (2006). 1Experimen...
