Agilent Technologies BL21-Gold Competent Cells, BL21-Gold(DE3)Competent Cells, and BL21-Gold(DE3)pLysS Competent Cells Instruction Manual
Summary
Essential reagent for advanced molecular biology research, these BL21-Gold Competent Cells support safe and efficient bacterial transformation for cloning and protein expression studies. The accompanying manual is an indispensable resource for scientists, providing detailed protocols on cell handling, media preparation, induction techniques using IPTG or Lambda CE6, and troubleshooting guides to ensure repeatable results in the lab. Ideal for molecular biology researchers conducting genetic modification research.
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BL21-Gold Competent Cells, BL21-Gold(DE3) Competent Cells, and BL21- Gold(DE3)p Lys S Competent Cells
Instruction Manual Catalog #230130 (BL21-Gold Competent Cells), #230132 [BL21-Gold(DE3) Competent Cells], and #230134 [BL21-Gold(DE3)p Lys S Competent Cells] Revision B
Research Use Only. Not for Use in Diagnostic Procedures. 230130-12
Page Summary Contents For Agilent Technologies BL21-Gold Competent Cells, BL21-Gold(DE3)Competent Cells, and BL21-Gold(DE3)pLysS Competent Cells Instruction Manual
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BL21-Gold Competent Cells, BL21-Gold(DE3) Competent Cells, and BL21- Gold(DE3)p Lys S Competent Cells Instruction Manual Catalog #230130 (BL21-Gold Competent Cells), #230132 [BL21-Gold(DE3) Competent ...
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LIMITED PRODUCT WARRANTY This warranty limits our liability to replacement of this product. No other warranties of any kind, express or implied, including without limitation, implied warranties of mer...
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BL21-Gold Competent Cells, BL21-Gold(DE3) Competent Cells, and BL21-Gold(DE3)p Lys S Competent Cells CONTENTS Materials Provided ..........................................................................
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BL21-Gold Competent Cells, BL21-Gold(DE3) Competent Cells, and BL21-Gold(DE3)p Lys S Competent Cells MATERIALS PROVIDED Catalog number Efficiency (cfu/μg of p UC18 Materials provided Tube color #23013...
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ACADEMIC AND NONPROFIT LABORATORY ASSURANCE LETTER The T7 expression system is based on technology developed at Brookhaven National Laboratory under contract with the U.S. Department of Energy and is ...
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INTRODUCTION BL21-Gold competent cells, BL21-Gold(DE3) competent cells, and BL21- Gold(DE3)p Lys S competent cells are improved versions of BL21 competent cells.3 These high-performance competent cell...
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Features We offer three different competent cell lines that provide varying levels of expression control with T7 promoter-driven vectors such as the p CAL vectors‡ and the p ET vectors.‡ BL21-Gold(DE3...
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TRANSFORMATION GUIDELINES Important For optimal transformation efficiency, please read the guidelines outlined in the following sections before proceeding with the Transformation Protocol. Storage Con...
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TRANSFORMATION PROTOCOL 1. Thaw the competent cells on ice. Note Store the competent cells on ice at all times while aliquoting. It is essential that the 14-ml BD Falcon polypropylene round- bottom tu...
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Transformation Summary for the p UC18 Control Plasmid Expected Efficiency Plating number of (cfu/μg of Host strain quantity colonies p UC18 DNA) BL21-Gold competent cells 5 μl >50 ≥1 × 108 BL21-Gol...
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Note These values for IPTG concentration and induction time are starting values only and may require optimization depending on the gene expressed. 5. After the induction period, place the cultures on ...
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5. Flood each plate with 5 ml of SM solution (see Preparation of Media and Reagents) and rock the plates for 2 hours at room temperature. 6. Remove the SM solution (which contains the lambda CE6) from...
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5. Grow the culture to an A600 of 0.6–1.0 and then add Mg SO4 to a final concentration of 10 m M (e.g. 500 μl of a 1.0 M solution of Mg SO4 to the 50-ml culture). 6. Remove a portion of the culture to...
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PREPARATION OF MEDIA AND REAGENTS LB Broth (per Liter) LB Agar (per Liter) 10 g of Na Cl 10 g of Na Cl 10 g of tryptone 10 g of tryptone 5 g of yeast extract 5 g of yeast extract 20 g of agar Add d H2...
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(table continues from previous page) SM Solution NZY Broth, (per Liter) 5 g of Na Cl 5 g of Na Cl 2 g of Mg SO4 . 7H2O 2 g of Mg SO4 . 7H2O 50 ml of 1 M Tris-HCl (p H 7.5) 5 g of yeast extract 5 ml 2%...
Manual Details
| Brand | Agilent Technologies |
|---|---|
| Pages | 15 |
| File Size | 227.90 KB |
| Published | May 13, 2026 |
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Frequently Asked Questions
What are the catalog numbers for the different BL21-Gold strains?
The catalog numbers are #230130 for BL21-Gold, #230132 for BL21-Gold(DE3), and #230134 for BL21-Gold(DE3)pLysS.
What precaution must be taken before using the sample buffer for denaturing gels?
You must add dithiothreitol (dtt) to a final concentration of 200 mM in the 2× buffer prior to use.
Where can I access Material Safety Data Sheets (MSDS) for this product?
MSDSDocuments are not included with shipments but are provided online at http://www.genomics.agilent.com.
Does Agilent provide a guarantee against all types of damages?
No, the limited warranty only limits liability to replacement of the product and excludes indirect or consequential damages.