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Agilent Technologies Perfect Match PCR Enhancer Instruction Manual

Summary

Enhance your molecular biology research with this specialized PCR additive, designed to significantly boost both the specificity and yield of primary amplification products. Perfect Match PCR Enhancer stabilizes primer-template complexes, drastically reducing false priming artifacts commonly encountered when working with genomic or plasmid DNA templates. The accompanying manual provides detailed guidance for researchers, covering optimized dosage ratios, complete procedural guidelines, and essential troubleshooting steps to ensure highly specific and reproducible results in your laboratory work.

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Perfect Match PCR Enhancer

Instruction Manual Catalog #600129 (100 U) and #600130 (200 U) Revision B

Research Use Only. Not for Use in Diagnostic Procedures. 600129-12

Page Summary Contents For Agilent Technologies Perfect Match PCR Enhancer Instruction Manual

Page 1 Perfect Match PCR Enhancer Instruction Manual Catalog #600129 (100 U) and #600130 (200 U) Revision B Research Use Only. Not for Use in Diagnostic Procedures. 600129-12
Page 2 LIMITED PRODUCT WARRANTY This warranty limits our liability to replacement of this product. No other warranties of any kind, express or implied, including without limitation, implied warranties of mer...
Page 3 Perfect Match PCR Enhancer CONTENTS Contents ............................................................................................................................................... 3  Material...
Page 4 Perfect Match PCR Enhancer MATERIALS PROVIDED Quantity Material provided Catalog #600129 Catalog #600130 Perfect Match PCR enhancer (1U/μl) 100 U 200 U STORAGE CONDITIONS Perfect Match PCR Enhancer: –...
Page 5 INTRODUCTION Perfect Match PCR enhancer* is an additive that increases the specificity and yield of primary PCR amplification products. When added to genomic in vitro amplification reactions, Perfect ...
Page 6 AMPLIFICATION PROTOCOL Note The following protocol is designed for one primer extension amplification reaction. 1. To prepare the amplification reaction, add the following components to a 0.5-ml micro...
Page 7 7. Program the thermal cycler for 30 cycles using the cycling parameters indicated in the following table: Cycle Temperature Duration Extension 72°C 30–60 seconds/kb Melting 94–95°C 1 minute Annealing...

Manual Details

Brand Agilent Technologies
Pages 7
File Size 156.79 KB
Published May 13, 2026
97 views

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Frequently Asked Questions

What is the primary function of the Perfect Match PCR enhancer?

It is an additive that increases the specificity and yield of primary PCR amplification products.

How should I store the Perfect Match PCR Enhancer product?

Store it at –20°C.

What is the recommended ratio for plasmid template optimization?

Use 1 U of Perfect Match PCR enhancer per μg of DNA template.

If I use primers less than 17 mers, what should I adjust in the protocol?

The annealing temperature must be reduced.