Agilent MassHunter Workstation Software - Data Acquisition for 6410 Triple Quad LC MS

Agilent Mass Hunter Workstation Software – Data Acquisition for 6410 Triple Quad LC/MS Familiarization Guide Before you begin 3 Prepare your system 3 Prepare to acquire data 3 Exercise – Develop an acquisition method for the 6410 5 Task 1. Enter acquisition parameters and acquire data 5 Task 2. Determine precursor ion masses 10 Task 3. Find optimum fragmentor voltage for maximum response 13 Task 4. Determine product ion masses 23 Task 5. Find optimum collision energy for MRM acquisition 30

Use the exercise in this guide to learn to use the Agilent 6410 Triple Quad LC/MS. You can do these exercises with the demo data files, Sulfa Drugs shipped with the system (in the Data folder of your Data Acquisition instal- lation disk), or with data you acquire.

With this exercise, you learn how to determine the best acquisition settings for analyzing your compounds of interest. These instructions help you understand not only how to set up a worklist to optimize instrument parameters for best sensitivity in acquisition, but also how to use the Qualitative Analysis program to identify parameter values producing optimum signal response. You can also learn about the Qualitative Analysis program by using the Qualitative Analysis Familiarization Guide.

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