Agilent Mass Hunter Software Quick Start Guide
Summary
Mass Hunter Software is an essential analytical tool designed for high-resolution mass spectral analysis, processing chromatographic and electrophoretic mass data. It automatically extracts critical molecular features, reduces complex data noise, and eliminates interferences to provide detailed lists of chemical components. This manual provides users with a comprehensive overview and practical tutorials on how to extract, review, compare, and export feature information from Agilent TOF files, allowing scientists to streamline their analytical workflow efficiently.
Page 1 Text Content
Agilent Mass Hunter Software Quick Start Guide Use this guide to get started with the Mass Hunter software.
What is Mass Hunter Software?
Mass Hunter is an integral part of Agilent TOF software (version A.02.00). Mass Hunter operates on chromatographic and electrophoretic mass spectral data to extract information to reduce data complexity, eliminate potential interferences, and generate a list of molecular features.
Working with the extracted information
A feature is a discrete molecular You can use the feature information from Mass Hunter to perform
entity defined by the combination many different tasks. Here are a few examples:
of retention time and mass. Compare the original (raw data) total ion chromatogram (TIC)
with the processed TIC Compare the original and processed mass spectra at a specific retention time (RT), or averaged over an RT range, on the TIC Show extracted ion chromatograms based on a mass spectral range. View the species clusters (isotopic, dimers, adducts) for each feature For a complete list of tasks, see the Mass Hunter online help.
Agilent Technologies
Page Summary Contents For Agilent Mass Hunter Software Quick Start Guide
Manual Details
| Brand | Hunter |
|---|---|
| Pages | 28 |
| File Size | 1.51 MB |
| Published | May 22, 2026 |
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Frequently Asked Questions
How do I access the Mass Hunter software?
Double-click the Agilent TOF Software folder icon on the desktop, or select Start > Programs > Agilent > TOF Software > Mass Hunter.
What data formats must be used for processing TOF results?
When loading .wiff files, they must be local to the software and cannot be read-only.
What is the primary function of Mass Hunter?
It extracts molecular features from mass spectral data to reduce complexity, eliminate interferences, and generate necessary molecular lists.
How can I view a 2D representation of my data?
The contour plot provides this by using m/z as the x-axis and RT as the y-axis (with intensity represented by darkness).